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KMID : 0383820100690060456
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Tuberculosis and Respiratory Diseases
2010 Volume.69 No. 6 p.456 ~ p.464
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The Effect of Gefitinib on Immune Response of Human Peripheral Blood Monocyte-Derived Dendritic Cells
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Cho Jin-Hoon
Kim Mi-Hyun
Lee Kwang-Ha
Kim Ki-Uk
Jeon Doo-Soo
Park Hye-Kyung
Kim Yun-Seong
Lee Min-Ki
Park Soon-Kew
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Abstract
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Background : Synergistic antitumor effects of the combined chemoimmunotherapy based on dendritic cells have been reported recently. The aim of this study is to search new applicability of gefitinib into the combination treatment through the confirmation of gefitinib effects on the monocyte derived dendritic cells (moDCs); most potent antigen presenting cell (APC).
Materials and Methods : Immature and mature monocyte-derived dendritic cell (im, mMoDC)s were generated from peripheral blood monocyte (PBMC) in Opti-MEM culture medium supplemented with IL-4, GM-CSF and cocktail, consisting of TNF-α (10 ng/mL), IL-1β (10 ng/mL), IL-6 (1,000 U/mL) and PGE2 (1 μ/mL). Various concentrations of gefitinib also added on day 6 to see the influence on immature and mature MoDCs. Immunophenotyping of DCs under the gefitinib was performed by using monoclonal antibodies (CD14, CD80, CD83, CD86, HLA-ABC, HLA-DR). Supernatant IL-12 production and apoptosis of DCs was evaluated. And MLR assay with [3H]-thymidine uptake assay was done.
Results : Expression of CD83, MHC I were decreased in mMoDCs and MHC I was decreased in imMoDCs under gefitinib. IL-12 production from mMoDCs was decreased under 10 μM of gefitinib sinificantly. Differences of T cell proliferation capacity were not observed in each concentration of geftinib.
Conclusion : In spite of decreased expressions of some dendritic cell surface molecules and IL-12 production under 10 μM of gefitinib, significant negative influences of gefitinib in antigen presenting capacity and T cell stimulation were not observed.
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KEYWORD
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Dendritic cells, gefitinib, Interleukin-12, Apoptosis
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